Journal: iScience

Article Title: Iloprost requires the Frizzled-9 receptor to prevent lung cancer

doi: 10.1016/j.isci.2022.104442

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Article Snippet: Ki67+ nuclei were detected using Betazoid DAB chromagen kit (Vector Laboratories BDB2004).

Techniques: Recombinant, Western Blot, Plasmid Preparation, Electrophoresis, Reporter Assay, BIA-KA, Immunodetection, Knock-Out, Transgenic Assay, Software

Journal: Cancer cell

Article Title: Multi-omic analyses of changes in the tumor microenvironment of pancreatic adenocarcinoma following neoadjuvant treatment with anti-PD-1 therapy

doi: 10.1016/j.ccell.2022.10.001

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Article Snippet: ImmPRESS DAB peroxidase substrate Kit , Vector Laboratories , Cat#SK-4105.

Techniques: Multiplex Assay, Immunohistochemistry, Recombinant, Staining, Plasmid Preparation, RNA HS Assay, Sequencing, Software, Cytometry

Journal: Cell reports

Article Title: Nanoparticle-based itaconate treatment recapitulates low-cholesterol/low-fat diet-induced atherosclerotic plaque resolution

doi: 10.1016/j.celrep.2024.114911

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Article Snippet: ImmPACT ® DAB EqV Substrate Kit, Peroxidase (HRP) , Vector Laboratories , SK-4103.

Techniques: Purification, Plasmid Preparation, Produced, Recombinant, Concentration Assay, Saline, Labeling, Membrane, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Phospholipid Assay, BIA-KA, RNAscope, HD Assay, Polymer, Sequencing, Expressing, Software, Microscopy

Journal: Cell reports

Article Title: Hippocampal Interneuronal α7 nAChRs Modulate Theta Oscillations in Freely Moving Mice

doi: 10.1016/j.celrep.2020.107740

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti dsred antibody Clonetech Cat# 632496; RRID:AB_10013483 biotinylated goat anti-rabbit secondary antibody Vector Laboratories Cat# BA-1000; RRID:AB_2313606 Bacterial and Virus Strains pAAV-EF1a-DIO- hChR2(H134R)-mCherry-WPRE-HGHpA Witten et al., 2010 addgene #20297 pAAV-EF1a-DIO-mCherry-WPRE-HGHpA Witten et al., 2010 addgene #20299 Chemicals, Peptides, and Recombinant Proteins methyllycaconitine Tocris Cat# 1029 dihydro-β-erythroidine Tocris Cat# 2349 atropine Sigma Cat# A0132 acetylcholine Sigma Cat# A6625 choline Sigma Cat# C7527 Critical Commercial Assays BaseScope Duplex Detection Reagent kit Advanced Cell Diagnostics Cat# 323810 RnaScope Duplex Detection Reagent kit Advanced Cell Diagnostics Cat# 322500 Vectastain Elite ABC kit Vector Laboratories Cat# PK-6100 DAB Peroxidase Substrate Kit Vector Laboratories Cat# SK-4100 Experimental Models: Organisms/Strains Mouse: floxed α7 nAChR (B6(Cg)-Chrna7tm1.1Ehs/YakelJ) The Jackson Laboratory Cat# 026965 Mouse: Gad2-cre (B6N.Cg-Gad2tm2(cre) Zjh/J) The Jackson Laboratory Cat# 019022 Mouse: CaMK2a-cre (B6.Cg-Tg(Camk2a-cre)T29–1Stl/J) The Jackson Laboratory Cat# 005359 Mouse: ChAT-cre (B6;129S6-Chattm2(cre) Lowl/J) The Jackson Laboratory Cat# 006410 Mouse: Sst-cre (Sst tm2.1(cre)Zjh) The Jackson Laboratory Cat# 013044 Mouse: Rosa tdTomato (B6.Cg-Gt(ROSA) 26Sor tm14(CAG-tdTomato)Hze/J) The Jackson Laboratory Cat# 007914 Mouse: OLM α2-cre mice (Tg(Chrna2cre) OE29Gsat/Mmucd) Mutant Mouse Resource and Research Centers Cat# 036704-UCD Oligonucleotides α7 nAChR mRNA probe Advanced Cell Diagnostics Cat# 724751-C2 Sst mRNA probe Advanced Cell Diagnostics Cat# 712861 GAD2 mRNA probe Advanced Cell Diagnostics Cat# 714361 CaMK2a mRNA probe Advanced Cell Diagnostics Cat# 724771 α2 nAChR mRNA probe Advanced Cell Diagnostics Cat# 416881 Software and Algorithms NeuroExplorer Nex Technologies https://www.neuroexplorer.com/ EthoVision Noldus https://www.noldus.com/ethovision-xt Cerebus acquisition system Blackrock Microsystems https://www.blackrockmicro.com/neuroscience-research-products/neural-data-acquisition-systems/cerebus-daq-system/ pClamp Molecular Devices https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite#gref Open in a separate window KEY RESOURCES TABLE Hippocampal α7 nAChRs regulate theta power in freely moving mice Hippocampal α7 nAChRs regulate Y-maze spontaneous alternation performance Interneuronal α7 nAChRs are critical for theta and Y-maze regulation

Techniques: Plasmid Preparation, Virus, Recombinant, Mutagenesis, Software

Journal: Cancer cell

Article Title: A p53 super-tumor suppressor reveals a tumor suppressive p53-Ptpn14-Yap axis in pancreatic cancer

doi: 10.1016/j.ccell.2017.09.007

Figure Lengend Snippet: DATA AND SOFTWARE AVAILABILITY

Article Snippet: Chromogenic staining was performed using the DAB peroxidase kit (Vector Laboratories) and the sections were counter-stained using hematoxylin (H-3401, Vector Laboratories).

Techniques: Software, Plasmid Preparation, Recombinant, Staining, Expressing

Journal: Cell

Article Title: Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury

doi: 10.1016/j.cell.2018.02.004

Figure Lengend Snippet:

Article Snippet: For antibodies raised in mouse, the M.O.M. kit (Vector Labs) was used following the manufacturer’s instructions.

Techniques: Plasmid Preparation, Recombinant, Protease Inhibitor, Imaging, Antibody Labeling, Bicinchoninic Acid Protein Assay, Sequencing, Software